Lionel Messi will be out for three weeks after suffering a groin strain in Barcelona’s draw with Atletico Madrid.
The striker went off on 59 minutes on Wednesday after a Diego Godin tackle.
He was forced to miss Argentina’s World Cup 2018 qualifier against Venezuela earlier this month with a groin injury, but has played in all five La Liga games for his club this season.
The Argentine could return for Barca’s Champions League tie with Manchester City on 19 October.
Prior to that tie, Messi will be ruled out of games against Borussia Monchengladbach in the Champions League, Sporting Gijon and Celta Vigo in La Liga, and World Cup qualifiers against Peru and Paraguay.
“Without Messi football loses but we have the squad to cope as we did when he was out last year,” said Luis Enrique.
Man City will hope the Barcelona forward, who has torn the abductor muscle in his right leg, returns rusty and not refreshed as they take on the Spanish champions pegged back by an intense Atletico Madrid side who look ready to play a big part in the title race this season.
Sergio Busquets was also forced off with injury in the second half of Wednesday’s game as Angel Correa’s equaliser cancelled out Ivan Rakitic’s opener at the Nou Camp.

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